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荧光染料及其激发和发射波长峰值如下表： 荧光染料示例 激发光 Indo-1, Ca saturated 331 nm Indo-1 Ca2+ 346 nm Cascade Blue BSA pH 7.0 401 nm Cascade Blue 398 nm LysoTracker Blue 373 nm Alexa 405 401 nm LysoSensor Blue pH 5.0 374 nm LysoSensor Blue 374 nm DyLight 405 399 nm DyLight 350 332 nm BFP (Blue Fluorescent Protein) 380 nm Alexa 350 343 nm 7-Amino-4-methylcoumarin pH 7.0 346 nm Amino Coumarin 345 nm AMCA conjugate 347 nm Coumarin 360 nm 7-Hydroxy-4-methylcoumarin 360 nm 7-Hydroxy-4-methylcoumarin pH 9.0 361 nm 6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0 358 nm Hoechst 33342 352 nm Pacific Blue 404 nm Hoechst 33258 352 nm Hoechst 33258-DNA 352 nm Pacific Blue antibody conjugate pH 8.0 404 nm PO-PRO-1 434 nm PO-PRO-1-DNA 435 nm POPO-1 433 nm POPO-1-DNA 433 nm DAPI-DNA 359 nm DAPI 358 nm Marina Blue 362 nm SYTOX Blue-DNA 445 nm CFP (Cyan Fluorescent Protein) 434 nm eCFP (Enhanced Cyan Fluorescent Protein) 437 nm 1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS) 375 nm Indo-1, Ca free 346 nm 1,8-ANS (1-Anilinonaphthalene-8-sulfonic acid) 375 nm BO-PRO-1-DNA 462 nm BOPRO-1 462 nm BOBO-1-DNA 461 nm SYTO 45-DNA 451 nm evoglow-Pp1 448 nm evoglow-Bs1 448 nm evoglow-Bs2 448 nm Auramine O 431 nm DiO 487 nm LysoSensor Green pH 5.0 447 nm Cy 2 489 nm LysoSensor Green 447 nm Fura-2, high Ca 336 nm Fura-2 Ca2+sup> 336 nm SYTO 13-DNA 488 nm YO-PRO-1-DNA 491 nm YOYO-1-DNA 491 nm eGFP (Enhanced Green Fluorescent Protein) 488 nm LysoTracker Green 503 nm GFP (S65T) 489 nm BODIPY FL, MeOH 502 nm Sapphire 396 nm BODIPY FL conjugate 503 nm MitoTracker Green 490 nm MitoTracker Green FM, MeOH 490 nm Fluorescein 0.1 M NaOH 493 nm Calcein pH 9.0 494 nm Fluorescein pH 9.0 490 nm Calcein 493 nm Fura-2, no Ca 367 nm Fluo-4 494 nm FDA 495 nm DTAF 495 nm Fluorescein 495 nm Fluorescein antibody conjugate pH 8.0 493 nm CFDA 495 nm FITC 495 nm Alexa Fluor 488 hydrazide-water 493 nm DyLight 488 493 nm 5-FAM pH 9.0 492 nm FITC antibody conjugate pH 8.0 495 nm Alexa 488 493 nm Rhodamine 110 497 nm Rhodamine 110 pH 7.0 497 nm Acridine Orange 431 nm Alexa Fluor 488 antibody conjugate pH 8.0 499 nm BCECF pH 5.5 485 nm PicoGreendsDNA quantitation reagent 502 nm SYBR Green I 498 nm Rhodaminen Green pH 7.0 497 nm CyQUANT GR-DNA 502 nm NeuroTrace 500/525, green fluorescent Nissl stain-RNA 497 nm DansylCadaverine 335 nm Rhodol Green antibody conjugate pH 8.0 499 nm Fluoro-Emerald 495 nm Nissl 497 nm Fluorescein dextran pH 8.0 501 nm Rhodamine Green 497 nm 5-(and-6)-Carboxy-2', 7'-dichlorofluorescein pH 9.0 504 nm DansylCadaverine, MeOH 335 nm eYFP (Enhanced Yellow Fluorescent Protein) 514 nm Oregon Green 488 498 nm Oregon Green 488 antibody conjugate pH 8.0 498 nm Fluo-3 506 nm BCECF pH 9.0 501 nm SBFI-Na+ 336 nm Fluo-3 Ca2+ 506 nm Rhodamine 123, MeOH 507 nm FlAsH 509 nm Calcium Green-1 Ca2+ 506 nm Magnesium Green 507 nm DM-NERF pH 4.0 493 nm Calcium Green 506 nm Citrine 515 nm LysoSensor Yellow pH 9.0 335 nm TO-PRO-1-DNA 515 nm Magnesium Green Mg2+ 507 nm Sodium Green Na+ 507 nm TOTO-1-DNA 514 nm Oregon Green 514 512 nm Oregon Green 514 antibody conjugate pH 8.0 513 nm NBD-X 466 nm DM-NERF pH 7.0 509 nm NBD-X, MeOH 467 nm CI-NERF pH 6.0 513 nm Alexa 430 431 nm Alexa Fluor 430 antibody conjugate pH 7.2 431 nm CI-NERF pH 2.5 504 nm Lucifer Yellow, CH 428 nm LysoSensor Yellow pH 3.0 389 nm 6-TET, SE pH 9.0 521 nm Eosin antibody conjugate pH 8.0 525 nm Eosin 524 nm 6-Carboxyrhodamine 6G pH 7.0 526 nm 6-Carboxyrhodamine 6G, hydrochloride 525 nm Bodipy R6G SE 528 nm BODIPY R6G, MeOH 528 nm 6 JOE 520 nm Cascade Yellow antibody conjugate pH 8.0 399 nm Cascade Yellow 399 nm mBanana 540 nm Alexa Fluor 532 antibody conjugate pH 7.2 528 nm Alexa 532 528 nm Erythrosin-5-isothiocyanate pH 9.0 533 nm 6-HEX, SE pH 9.0 534 nm mOrange 548 nm mHoneydew 478 nm Cy 3 549 nm Rhodamine B 543 nm DiI 551 nm 5-TAMRA-MeOH 543 nm Alexa 555 553 nm Alexa Fluor 555 antibody conjugate pH 7.2 553 nm DyLight 549 555 nm BODIPY TMR-X, SE 544 nm BODIPY TMR-X, MeOH 544 nm PO-PRO-3-DNA 539 nm PO-PRO-3 539 nm Rhodamine 551 nm Bodipy TMR-X conjugate 544 nm POPO-3 533 nm Alexa 546 562 nm BODIPY TMR-X antibody conjugate pH 7.2 544 nm Calcium Orange Ca2+ 549 nm TRITC 550 nm Calcium Orange 549 nm Rhodaminephalloidin pH 7.0 558 nm MitoTracker Orange 551 nm MitoTracker Orange, MeOH 551 nm Phycoerythrin 565 nm Magnesium Orange 550 nm R-Phycoerythrin pH 7.5 565 nm 5-TAMRA pH 7.0 553 nm 5-TAMRA 549 nm Rhod-2 552 nm FM 1-43 472 nm Rhod-2 Ca2+ 553 nm Tetramethylrhodamine antibody conjugate pH 8.0 552 nm FM 1-43 lipid 473 nm LOLO-1-DNA 568 nm dTomato 554 nm DsRed 563 nm Dapoxyl (2-aminoethyl) sulfonamide 372 nm Tetramethylrhodamine dextran pH 7.0 555 nm Fluor-Ruby 554 nm Resorufin 571 nm Resorufin pH 9.0 571 nm mTangerine 568 nm LysoTracker Red 578 nm Lissaminerhodamine 572 nm Cy 3.5 578 nm Rhodamine Red-X antibody conjugate pH 8.0 573 nm Sulforhodamine 101, EtOH 578 nm JC-1 pH 8.2 593 nm JC-1 592 nm mStrawberry 575 nm MitoTracker Red 578 nm MitoTracker Red, MeOH 578 nm X-Rhod-1 Ca2+ 580 nm Alexa Fluor 568 antibody conjugate pH 7.2 579 nm Alexa 568 576 nm 5-ROX pH 7.0 578 nm 5-ROX (5-Carboxy-X-rhodamine, triethylammonium salt) 578 nm BO-PRO-3-DNA 574 nm BOPRO-3 574 nm BOBO-3-DNA 570 nm Ethidium Bromide 524 nm ReAsH 597 nm Calcium Crimson 589 nm Calcium Crimson Ca2+ 590 nm mRFP 585 nm mCherry 587 nm Texas Red-X antibody conjugate pH 7.2 596 nm HcRed 590 nm DyLight 594 592 nm Ethidium homodimer-1-DNA 528 nm Ethidiumhomodimer 528 nm Propidium Iodide 538 nm SYPRO Ruby 467 nm Propidium Iodide-DNA 538 nm Alexa 594 590 nm BODIPY TR-X, SE 588 nm BODIPY TR-X, MeOH 588 nm BODIPY TR-X phallacidin pH 7.0 590 nm Alexa Fluor 610 R-phycoerythrin streptavidin pH 7.2 567 nm YO-PRO-3-DNA 613 nm Di-8 ANEPPS 469 nm Di-8-ANEPPS-lipid 469 nm YOYO-3-DNA 612 nm Nile Red-lipid 553 nm Nile Red 559 nm DyLight 633 624 nm mPlum 587 nm TO-PRO-3-DNA 642 nm DDAO pH 9.0 648 nm Fura Red, high Ca 434 nm Allophycocyanin pH 7.5 651 nm APC (allophycocyanin) 650 nm Nile Blue, EtOH 631 nm TOTO-3-DNA 642 nm Cy 5 646 nm BODIPY 650/665-X, MeOH 646 nm Alexa Fluor 647 R-phycoerythrin streptavidin pH 7.2 569 nm DyLight 649 652 nm Alexa Fluor 647 antibody conjugate pH 7.2 653 nm Alexa 647 653 nm Fura Red Ca2+ 435 nm Atto 647 644 nm Fura Red, low Ca 472 nm Carboxynaphthofluorescein pH 10.0 600 nm Alexa 660 664 nm Alexa Fluor 660 antibody conjugate pH 7.2 663 nm Cy 5.5 673 nm Alexa Fluor 680 antibody conjugate pH 7.2 679 nm Alexa 680 679 nm DyLight 680 678 nm Alexa Fluor 700 antibody conjugate pH 7.2 696 nm Alexa 700 696 nm FM 4-64, 2% CHAPS 506 nm FM 4-64 508 nm