DBCO-PEG-COOH,DBCO-PEG-ACID
作者:DBCO-PEG-COOH,DBCO-PEG-ACID 发布时间:2022-08-01 11:15:17
次浏览
名称
货号
英文名、缩写
分子量
规格
价格
DBCO-PEG-COOH
PS2-CMDB-5K
DBCO-PEG-COOH
5000
0.1g、0.5g
现询
DBCO-PEG-COOH
PS2-CMDB-2K
DBCO-PEG-COOH
2000
0.1g、0.5g
现询
DBCO-PEG-COOH
PS2-CMDB-3K
DBCO-PEG-COOH
3400
0.1g、0.5g
现询
DBCO-PEG-COOH
PS2-CMDB-1K
DBCO-PEG-COOH
1000
0.1g、0.5g
现询
DBCO-PEG-COOH
PS2-CMDB-10K
DBCO-PEG-COOH
10000
0.1g、0.5g
现询
(备注:非常规分子量:400、600。精确聚合度的小分子定制。)
结构式:
基本信息:
中文名称:Dibenzocyclooctyne-聚乙二醇-羧基 (DBCO-PEG-COOH)
别名:DBCO-PEG-COOH
英文名称:DBCO-PEG-COOH,DBCO-PEG-ACID
CAS:-
分子量:400、600、1K、2K、3.4K、4K、5K、10K
纯度:95%以上
应用简介:
’DBCO(dibenzocyclooctyne) PEG acid, DBCO-PEG-COOH, is a heterobifunctional reactivePEG derivative that can go Click Chemistry reaction without a need of any metalcatalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes andazides, also termed as the Cu-free click reaction, is a bioorthogonal reactionthat enables the conjugation of two molecules in aqueous solution. DBCO PEG derivativespossess fast kinetics and stability in aqueous buffer. DBCO reagents can beused to label azide-modified biomolecules spontaneously without the need fortoxic Cu catalysts. Carboxylic acid group, on the other hand, can be activatedto react with amines, hydroxy and other functional groups.
Copper-freeClick reaction procedures:
Preparethe azide-containing reagents in reaction buffer.
AddDBCO reagents to azide containing reagents. Add 1 mol equivalent of limitingreagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubatethe reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purifyconjugates either by dialysis or size-exclusion chromatograph.
物理性质:
状态:淡黄色到白色固体,或者半固体,取决于分子量。
溶解性:溶于水,氯仿,DMSO等溶剂。
保持条件:-20℃长期保存,避光,干燥。
注意事项:取用一定要干燥,避免频繁的溶解和冻干。
反应性:DBCO于含有N3基团的反应。NHS可以和NH2。
技术指标:
纯度(PEG):95%以上
取代率(DBCO):90%以上
取代率(COOH):95%以上
分散系数(PDI):小于等于1.05
分析:COA、GPC、NMR、MALDI-TOF