Cy5 Picolyl Azide,Cy5-N3,Alexa Fluor 647
产品介绍
Cy5 Picolyl Azide
Cy5 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concen*ration of Cu(I) at the reaction site. The chelating effect of picolyl azide moiety to raise the effective concen*ration of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold.
In addition, the use of Cy5 Picolyl Azide instead of conventional Cy5-Azide allows for at least a tenfold reduction in the concen*ration of the copper catalyst without sacrificing the efficiency of labeling.
In summary, the in*roduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.
Abs/Em Maxima
647/663 nm
Extinction coefficient
250,000
Flow cytometry laser line
633 or 635 nm
Microscopy laser line
633 or 635 nm
Spectrally similar dyes
Alexa Fluor® 647, Atto™ 647, CF™ 647 Dye, DyLight™649
Molecular weight
953.15
CAS
N/A
Solubility
Water, DMSO, DMF, MeOH
Purity
>95% (HPLC)
Appearance
Blue solid
Storage conditions
-20C
Shipping conditions
Ambient temperature
结构式:
Cy5 Picolyl Azide,Cy5-N3,Alexa Fluor 647
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炔烃 Dye alkynes
1-Ethynyl pyrene
3-Ethynyl perylene
BDP FL alkyne
FAM alkyne, 5-isomer
FAM alkyne, 6-isomer
TAMRA alkyne, 5-isomer
BDP TMR alkyne
Cyanine3 alkyne
Cyanine5 alkyne
Cyanine5.5 alkyne
Cyanine7 alkyne
Cyanine7.5 alkyne
Sulfo-Cyanine3 alkyne
Sulfo-Cyanine5 alkyne