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5-羧基四甲基罗丹明琥珀酰亚胺,150810-68-7
Although the mixed isomers of 5(6)-TAMRA, SE, also know as 5(6)-TAMRA NHS Ester is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides, purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominay used for labeling nucleotides and sequencing nucleic acids. 5-TAMRA dye is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.
结构式：
5-羧基四甲基罗丹明琥珀酰亚胺,150810-68-7
Abs/Em Maxima
545/576 nm
Extinction coefficient
78,000
Flow cytometry laser line
532 555 or 568 nm
Microscopy laser line
532 or 555 nm
Spectrally similar dyes
Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549
Molecular weight
527.53
CAS
150810-68-7
Solubility
DMSO, DMF, MeOH
Purity
>95% (HPLC)
Appearance
Dark red solid
Storage conditions
-20°C. Desiccate
Shipping conditions
Ambient temperature
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