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5-TAMRA Azide|5-羧甲基罗丹明叠氮
5-TAMRA Azide (also known as TAMRA azide, isomer 5) is the red-fluorescent probe that is patible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free &lduo;click chemistry&rduo; reaction to form a stable triazole and does not reuire Cu-catalyst or elevated temperatures.
Although the mixed isomers of 5(6)-TAMRA azide is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often reuired in the conjugation processes.
5-TAMRA azide is exact replacement of Invitrogen&rsuo;s (Thermo Fisher Scientific) Tetramethylrhodamine (TAMRA) Azide (Tetramethylrhodamine 5-Carboxamido-(6-Azidohexanyl)), 5-isomer
     
Abs/Em Maxima
553/575
Extinction coefficient
92,000
Flow cytometry laser line
532 or 555 nm
Microscopy laser line
532 or 555 nm
Spectrally similar dyes
Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549
Molecular weight
555.67
CAS
1006592-61-5
Solubility
Water, DMSO, DMF, MeOH
Purity
>98% (HPLC)
Appearance
Red solid
Storage conditions
-20C
Shipping conditions
Ambient temperature
5-TAMRA Azide|5-羧甲基罗丹明叠氮
其他染料列表：
5(6)-Carboxy tetramethyl rhodamine;5(6)-TAMRA  150347-56-1
5-Carboxytetramethylrhodamine; 5-TAMRA  91809-66-4
6-Carboxytetramethylrhodamine;6-TAMRA  91809-67-5
5(6)-Carboxytetramethylrhodamine succinimidyl ester;5(6)-TAMRA, SE 246256-50-8
5-Carboxytetramethylrhodamine succinimidyl ester;5-TAMRA, SE  150810-68-7
6-Carboxytetramethylrhodamine succinimidyl ester;6-TAMRA, SE  150810-69-8
1,4-dichloro 5-Carboxytetramethylrhodamine  198546-47-3
1,4-dichloro 6-Carboxytetramethylrhodamine  407581-83-3
5-Carboxy-X- rhodamine; 5-ROX   216699-35-3
6-Carboxy-X- rhodamine; 6-ROX   194785-18-7
5(6)-Carboxy-X- rhodamine; 5(6)-ROX    198978-94-8
5(6)-ROX, SE [5-(6)-Carboxy-X-rhodamine, succinimidyl ester] 
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] 
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester]
Rhodamine 101;Rhodamine 640    64339-18-0
Rhodamine 110 chloride   13558-31-1
Rhodamine 123   62669-70-9
6-ROX C2 Maleimide 
6-ROX alkyne
6-ROX azide
5(6)-TAMRA Maleimide 
5-TAMRA Maleimide
6-TAMRA Maleimide 
5-TAMRA alkyne
6-TAMRA alkyne 
5-TAMRA azide
6-TAMRA azide
5(6)-TAMRA cadaverine
5-TAMRA cadaverine 
6-TAMRA cadaverine 
5(6)-TAMRA ethylenediamine
5-TAMRA ethylenediamine 
6-TAMRA ethylenediamine
6-Aminotetramethylrhodamine (6-氨基四甲基罗丹明)  80724-18-1
5-Aminotetramethylrhodamine  (5-氨基四甲基罗丹明)  80724-17-0,167095-10-5
5-TAMRA cadaverine
5-TAMRA NHS Ester                                  150810-68-7
5(6)-TAMRA NHS Ester                               150408-83-6
TAMRA Acid                                         98181-63-6
5-Carboxy Fluorescein; 5-FAM  5-羧基荧光素         76823-03-5
6-Carboxy Fluorescein; 6-FAM  6-羧基荧光素 3301-79-9
5(6)Carboxy fluorescein; 5(6)-FAM  5(6)羧基荧光素72088-94-9
1,4-dichloro 5-Carboxytetramethylrhodamine         198546-47-3
1,4-dichloro 6-Carboxytetramethylrhodamine         407581-83-3
Sulforhodamine 101; Sulforhodamine 640             60311-02-6
5-Carboxy-X-rhodamine; 5-ROX                       216699-35-3
6-Carboxy-X-rhodamine; 6-ROX                       194785-18-7
5(6)-Carboxy-X-rhodamine; 5(6)-ROX                 198978-94-8
Rhodamine 101;Rhodamine 640                        64339-18-0
Cy2                                                260430-02-2
Cy2-SE;Cy2 NHS Ester                               1205-33-4
Cy3                                                146368-13-0
Cy3-SE;Cy3 NHS Ester                               146368-16-3
Cy5                                                146368-11-8
Cy5-SE;Cy5 NHS Ester                            146368-14-1
Cy7                                                943298-08-6
Cy7-SE; Cy7 NHS Ester                              477908-53-5
Cy3-alkyne                                         10103-62-5
Cy5-alkyne                                         1345823-20-2
Cy7-alkyne
Calcein tetraethyl ester                           1170856-93-5
Dansyl chloride丹磺酰氯                          605-65-2
Dansyl acid                                        6268-49-1
DABCYL acid, SE                                    146998-31-4
DNP-X acid                                         10466-72-5
8-Hydroxy julolidine                               41175-50-2
3,6-Dichloro trimellitic acid                      137071-78-4
Boc-1,2-diaminoethane                              57260-73-8 
Boc-1,3-diaminobutane                              75178-96-0
Boc-1,4-diaminobutane                              68076-36-8
N-(4-Bromobutyl)phthalimide                        5394-18-3
2-[2-[2-(2-Bromoethoxy)ethoxy]ethoxy]-ethanol      85141-94-2
14-bromo-3,6,9,12-tetraoxatetradecan-1-ol          957205-14-0
Diethyl carboxymethylphosphonate                   3095-95-2
Indocyanine Green  ICG吲哚菁绿                   3599-32-4
PI（碘化丙啶）  Propidium Iodide                   25535-16-4